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nucleoband xtra bac large contract dna purification kit  (MACHEREY NAGEL)


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    Structured Review

    MACHEREY NAGEL nucleoband xtra bac large contract dna purification kit
    Nucleoband Xtra Bac Large Contract Dna Purification Kit, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 93/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/large+construct+dna+purification+kit/NucleoBond+Xtra+BAC+kit+for+large+construct+plasmid+DNA/bio_rxiv__2025__09__19__677276-206-4-12
    Average 93 stars, based on 39 article reviews
    nucleoband xtra bac large contract dna purification kit - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    DNA Purification:

    Article Title: Recombinant herpes simplex virus having expression cassette expressing fused protein of cancer cell-targeting domain and extracellular domain of HVEM and use thereof
    Article Snippet: .. The completed KOS-BAC-EmGFP-gD-R222N/F223I DNA was extracted using a large-construct DNA purification kit (Macherey-Nagel), after which 2×105 Cre-Vero-HVEM cells were transfected with 1 μg of DNA using a Lipofectamine 2000 reagent (Invitrogen). ..

    Article Title: Food and water intake are regulated by distinct central amygdala circuits revealed using intersectional genetics
    Article Snippet: .. Modified BAC DNA was prepared using the large construct DNA purification kit—NucleoBond® Xtra BAC (Macherey-Nagel), linearized with PmeI and purified through a SepharoseTM separation column . ..

    Article Title: Food and water intake are regulated by distinct central amygdala circuits revealed using intersectional genetics.
    Article Snippet: Plasmid construction and BACmodification was done by Gene Bridges GmbH. .. BACDNA for pronuclear injectionwasprepared using the large construct DNA purification kit—NucleoBond® Xtra BAC (MachereyNagel), linearized with PI-SceI and purified over a home-made Sepharose CL4B column (Johansson et al.75). .. Fractions were analyzed by pulsed-field gel electrophoresis to identify the sample with the highest concentration of linearized BAC DNA and lowest concentration of vector DNA.

    Article Title: Recombinant herpes simplex virus having expression cassette expressing fused protein of cancer cell-targeting domain and extracellular domain of HVEM and use thereof
    Article Snippet: .. Next, for viral production, the completed KOS-BAC-gD-R222N/F223I DNA was extracted using a large-construct DNA purification kit (Macherey-Nagel), after which 2×105 Cre-Vero-HVEM cells were transfected with 1 μg of DNA using a Lipofectamine 2000 reagent (Invitrogen). .. Next, for viral production, the completed KOS-BAC-gD-R222N/F223I DNA was extracted using a large-construct DNA purification kit (Macherey-Nagel), after which 2×105 Cre-Vero-HVEM cells were transfected with 1 μg of DNA using a Lipofectamine 2000 reagent (Invitrogen).

    Article Title: Identification of Spinal Neurons Contributing to the Dorsal Column Projection Mediating Fine Touch and Corrective Motor Movements
    Article Snippet: .. Modified BAC DNA was prepared using the large construct DNA purification kit, NucleoBond ® Xtra BAC (Macherey-Nagel), linearized with AscI and purified through a Sepharose separation column ( Johansson et al., 2010 ). ..

    Article Title: Food and water intake are regulated by distinct central amygdala circuits revealed using intersectional genetics
    Article Snippet: Plasmid construction and BAC modification was done by Gene Bridges GmbH. .. BAC DNA for pronuclear injection was prepared using the large construct DNA purification kit—NucleoBond® Xtra BAC (Macherey-Nagel), linearized with PI-SceI and purified over a home-made Sepharose CL4B column (Johansson et al. ). .. Fractions were analyzed by pulsed-field gel electrophoresis to identify the sample with the highest concentration of linearized BAC DNA and lowest concentration of vector DNA.

    Transfection:

    Article Title: Recombinant herpes simplex virus having expression cassette expressing fused protein of cancer cell-targeting domain and extracellular domain of HVEM and use thereof
    Article Snippet: .. The completed KOS-BAC-EmGFP-gD-R222N/F223I DNA was extracted using a large-construct DNA purification kit (Macherey-Nagel), after which 2×105 Cre-Vero-HVEM cells were transfected with 1 μg of DNA using a Lipofectamine 2000 reagent (Invitrogen). ..

    Article Title: Recombinant herpes simplex virus having expression cassette expressing fused protein of cancer cell-targeting domain and extracellular domain of HVEM and use thereof
    Article Snippet: .. Next, for viral production, the completed KOS-BAC-gD-R222N/F223I DNA was extracted using a large-construct DNA purification kit (Macherey-Nagel), after which 2×105 Cre-Vero-HVEM cells were transfected with 1 μg of DNA using a Lipofectamine 2000 reagent (Invitrogen). .. Next, for viral production, the completed KOS-BAC-gD-R222N/F223I DNA was extracted using a large-construct DNA purification kit (Macherey-Nagel), after which 2×105 Cre-Vero-HVEM cells were transfected with 1 μg of DNA using a Lipofectamine 2000 reagent (Invitrogen).

    Modification:

    Article Title: Food and water intake are regulated by distinct central amygdala circuits revealed using intersectional genetics
    Article Snippet: .. Modified BAC DNA was prepared using the large construct DNA purification kit—NucleoBond® Xtra BAC (Macherey-Nagel), linearized with PmeI and purified through a SepharoseTM separation column . ..

    Article Title: Identification of Spinal Neurons Contributing to the Dorsal Column Projection Mediating Fine Touch and Corrective Motor Movements
    Article Snippet: .. Modified BAC DNA was prepared using the large construct DNA purification kit, NucleoBond ® Xtra BAC (Macherey-Nagel), linearized with AscI and purified through a Sepharose separation column ( Johansson et al., 2010 ). ..

    BAC Assay:

    Article Title: Food and water intake are regulated by distinct central amygdala circuits revealed using intersectional genetics
    Article Snippet: .. Modified BAC DNA was prepared using the large construct DNA purification kit—NucleoBond® Xtra BAC (Macherey-Nagel), linearized with PmeI and purified through a SepharoseTM separation column . ..

    Article Title: Food and water intake are regulated by distinct central amygdala circuits revealed using intersectional genetics.
    Article Snippet: Plasmid construction and BACmodification was done by Gene Bridges GmbH. .. BACDNA for pronuclear injectionwasprepared using the large construct DNA purification kit—NucleoBond® Xtra BAC (MachereyNagel), linearized with PI-SceI and purified over a home-made Sepharose CL4B column (Johansson et al.75). .. Fractions were analyzed by pulsed-field gel electrophoresis to identify the sample with the highest concentration of linearized BAC DNA and lowest concentration of vector DNA.

    Article Title: Identification of Spinal Neurons Contributing to the Dorsal Column Projection Mediating Fine Touch and Corrective Motor Movements
    Article Snippet: .. Modified BAC DNA was prepared using the large construct DNA purification kit, NucleoBond ® Xtra BAC (Macherey-Nagel), linearized with AscI and purified through a Sepharose separation column ( Johansson et al., 2010 ). ..

    Article Title: Food and water intake are regulated by distinct central amygdala circuits revealed using intersectional genetics
    Article Snippet: Plasmid construction and BAC modification was done by Gene Bridges GmbH. .. BAC DNA for pronuclear injection was prepared using the large construct DNA purification kit—NucleoBond® Xtra BAC (Macherey-Nagel), linearized with PI-SceI and purified over a home-made Sepharose CL4B column (Johansson et al. ). .. Fractions were analyzed by pulsed-field gel electrophoresis to identify the sample with the highest concentration of linearized BAC DNA and lowest concentration of vector DNA.

    Construct:

    Article Title: Food and water intake are regulated by distinct central amygdala circuits revealed using intersectional genetics
    Article Snippet: .. Modified BAC DNA was prepared using the large construct DNA purification kit—NucleoBond® Xtra BAC (Macherey-Nagel), linearized with PmeI and purified through a SepharoseTM separation column . ..

    Article Title: Food and water intake are regulated by distinct central amygdala circuits revealed using intersectional genetics.
    Article Snippet: Plasmid construction and BACmodification was done by Gene Bridges GmbH. .. BACDNA for pronuclear injectionwasprepared using the large construct DNA purification kit—NucleoBond® Xtra BAC (MachereyNagel), linearized with PI-SceI and purified over a home-made Sepharose CL4B column (Johansson et al.75). .. Fractions were analyzed by pulsed-field gel electrophoresis to identify the sample with the highest concentration of linearized BAC DNA and lowest concentration of vector DNA.

    Article Title: Identification of Spinal Neurons Contributing to the Dorsal Column Projection Mediating Fine Touch and Corrective Motor Movements
    Article Snippet: .. Modified BAC DNA was prepared using the large construct DNA purification kit, NucleoBond ® Xtra BAC (Macherey-Nagel), linearized with AscI and purified through a Sepharose separation column ( Johansson et al., 2010 ). ..

    Article Title: Food and water intake are regulated by distinct central amygdala circuits revealed using intersectional genetics
    Article Snippet: Plasmid construction and BAC modification was done by Gene Bridges GmbH. .. BAC DNA for pronuclear injection was prepared using the large construct DNA purification kit—NucleoBond® Xtra BAC (Macherey-Nagel), linearized with PI-SceI and purified over a home-made Sepharose CL4B column (Johansson et al. ). .. Fractions were analyzed by pulsed-field gel electrophoresis to identify the sample with the highest concentration of linearized BAC DNA and lowest concentration of vector DNA.

    Purification:

    Article Title: Food and water intake are regulated by distinct central amygdala circuits revealed using intersectional genetics
    Article Snippet: .. Modified BAC DNA was prepared using the large construct DNA purification kit—NucleoBond® Xtra BAC (Macherey-Nagel), linearized with PmeI and purified through a SepharoseTM separation column . ..

    Article Title: Food and water intake are regulated by distinct central amygdala circuits revealed using intersectional genetics.
    Article Snippet: Plasmid construction and BACmodification was done by Gene Bridges GmbH. .. BACDNA for pronuclear injectionwasprepared using the large construct DNA purification kit—NucleoBond® Xtra BAC (MachereyNagel), linearized with PI-SceI and purified over a home-made Sepharose CL4B column (Johansson et al.75). .. Fractions were analyzed by pulsed-field gel electrophoresis to identify the sample with the highest concentration of linearized BAC DNA and lowest concentration of vector DNA.

    Article Title: Identification of Spinal Neurons Contributing to the Dorsal Column Projection Mediating Fine Touch and Corrective Motor Movements
    Article Snippet: .. Modified BAC DNA was prepared using the large construct DNA purification kit, NucleoBond ® Xtra BAC (Macherey-Nagel), linearized with AscI and purified through a Sepharose separation column ( Johansson et al., 2010 ). ..

    Article Title: Food and water intake are regulated by distinct central amygdala circuits revealed using intersectional genetics
    Article Snippet: Plasmid construction and BAC modification was done by Gene Bridges GmbH. .. BAC DNA for pronuclear injection was prepared using the large construct DNA purification kit—NucleoBond® Xtra BAC (Macherey-Nagel), linearized with PI-SceI and purified over a home-made Sepharose CL4B column (Johansson et al. ). .. Fractions were analyzed by pulsed-field gel electrophoresis to identify the sample with the highest concentration of linearized BAC DNA and lowest concentration of vector DNA.

    Injection:

    Article Title: Food and water intake are regulated by distinct central amygdala circuits revealed using intersectional genetics
    Article Snippet: Plasmid construction and BAC modification was done by Gene Bridges GmbH. .. BAC DNA for pronuclear injection was prepared using the large construct DNA purification kit—NucleoBond® Xtra BAC (Macherey-Nagel), linearized with PI-SceI and purified over a home-made Sepharose CL4B column (Johansson et al. ). .. Fractions were analyzed by pulsed-field gel electrophoresis to identify the sample with the highest concentration of linearized BAC DNA and lowest concentration of vector DNA.

    other:

    Article Title: Recombinant herpes simplex virus having expression cassette expressing fused protein of cancer cell-targeting domain and extracellular domain of HVEM and use thereof
    Article Snippet: Based on the principle whereby streptomycin resistance blocked by rpsL is activated while replacing the conventionally inserted UL3/4-rpsL-neo/kan cassette with the above inserted UL3/4-pCMV-Her2scFv-HveA-bGHpA, UL3/4-pCMV-HveA-Her2scFv-bGHpA and UL3/4-pCMV-CEAscFv-HveA-bGHpA, candidates were selected in a streptomycin medium (Heermann R et al., Microb Cell Fact.



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